Friday, October 31, 2014

Protein concentration, membrane based, Molecular Weight Cutoff aka Nominal Molecular Weight Limit

Protein concentrator based on membrane and centrifugation works by removing biomolecules at determined molecular size/weight. For example, a membrane-based protein concentrator such as Centriprep Centrifugal Filter Unit with Ultracel-50 membrane (Merck Millipore cat# 4311) has a protein cutoff point at 50 kDa. The term used can be  Molecular Weight Cutoff (MWCO; refer to Wikipedia) or Nominal Molecular Weight Limit (NMWL) but they mean similar thing, that proteins at or above 50 kDa is trapped/concentrated whereas molecules at <50 be="" br="" kda="" removed.="" will="">

Abstract

Writing abstract
If writers are able to conceive the detail of the abstract prior to writing other parts of a paper, thesis etc., it will be of great help. Abstracts can be considered as a mini-thesis (at around 100 words).

It should contain the following information:
  • What was done/what are the findings?
  • The reason?
  • The method?
  • The results?
  • The significance of finding?

References
Writing an abstract (link)

How to go about hard binding thesis in NTU Singapore

At last, finished my PhD defense and on the way to hard bind my thesis. Although NTU Library no longer collect hard copy theses, NTU schools still collect them for archiving. So, I need to prepare these hard copies.

To have my thesis bound in hard cover, I had to do it at the NTU library printing station (Campus Supplies Pte Ltd). The printing station is equipped with plethora of printers, scanners, and photocopiers. The payment method can be made via Ezlink. The shop was well equipped to cater to students, staffs and lecturers' needs.

One thing I didn't really fancy in the place. Not all staff is friendly. They are not rude, but they aren't welcoming and friendly. What a shame. The whole affair was very terse and impatient. Fortunately, not all the staff are like that. I guess their mundane work and the lack of friendlier and conducive environment sort of transformed them into zombie-like.

Anyway, the cost for hard binding a thesis is SGD 18. For two theses, it costs SGD 34. There is a submission date and a collection date (which is a week after submission). The theses are sent to a binder and collected in a week's time.

Alternatively, students can also contact another shop providing the above service, e.g. Ultra Supplies at Queensway. I have not contacted them because the place is far from NTU and probably nearer to students staying near NUS.

Contact
Ultra Supplies
No. 1 Queensway Office, #03-38/39
Main Service Counters, #03-37/45
Queensway Shopping Centre
Singapore 149053
Tel: 64796074
Fax: 64750845

For friendly assistance, call
Philip at 90214846
Kevin at 90216062

Campus Supplies Pte Ltd
Lee Wee Nam Library NTU
Tel: 67935980
Email: campusntu@campus-supplies.com

Mailing address:
Kent Ridge P. O. Box 1053
S911102

If given the chance, I would like to try dealing with Ultra Supplies and compare the service standard with that of Campus Supplies Pte Ltd. This is considering that the business card of the former included the description "for friendly assistance, call". That's a strange way to assure customers of friendly service. I would have expected all staffs should be friendly rather than assigning certain staffs as friendly...

Strange thought.