Monday, April 25, 2016

Vapor & liquid phase of liquid nitrogen storage

At liquid phase the temperature is -196oC and at the gaseous phase, the temperature is at the range of 180 to 190oC.


Ice crystallization leads to freezing damage of biological materials that consist of water. To preserve the material with high viability, it is crucial to suppress formation of ice crystals. To do this, it is best to achieve vitrification (or glass state), aka liquid in the fourth state which behaves like solid with high viscosity, thus impeding crystal movement and growth.


At -80oC water forms hexagonal crystal, -130oC is  water recrystallization point that forms cubic crystals, and -150oC is when water forms glass state.


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