For the type of pipettes, refer to this document available at cookmedical (micromanipulation pipette).
This is the first time I am going to handle the above assembly to manipulate embryos and I am researching the type of micromanipulation pipettes available.
In addition to getting pipettes from manufacturers, these pipettes can be made in the lab using a micropipette puller. Refer to Sutter pipette cookbook to determine the settings required for pulling different types of pipette. These pipettes need to be fire-polished (or flame-polished) to smoothen the surface.
In addition, holding pipette can be generated by briefly exposing micropipette to flame (or via microforge for more precision under microscope observation).
Fig shows fire-polished glass micropipette or pipette. Taken from Nature Publishing.
Fig shows microforge used for processing micropipettes. Taken from Tritechresearch.
A video showing how to process micropipette using microforge and grinder.
Equipment available in the following links
- Narishige needle processing catalog by Tritechresearch
- Sutter Instrument micropipettes fabrication tools.
- Leica DMi8 microscope for micromanipulation.
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Micromanipulation pipettes are all almost used in the laboratory by the laboratory personnel like microinjection, and in the use of the process, they will encounter some problems.
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